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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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HPLC QC Method: Determination of Crotamiton

  
  
  
  
  
  

cis & trans isomers in crotamiton This blog post discusses a more efficient quality control (QC) method for the determination of the cis and trans isomers that comprise crotamiton used in the treatment of scabies and skin pruritus than the official protocols of the British Pharmacopoeia (BP) and Pharmacopeia of the People’s Republic of China (PPRC).

Both the Pharmacopoeia specify that the maximum percentage of cis isomer should be 15% of the sum of cis and trans isomers, and have published normal-phase liquid chromatography methods for isomer analysis.

Application Brief 138, Separation of Cis and Trans Isomers of Crotamiton Using an Acclaim C30 Column, (downloadable PDF) describes a convenient reversed-phase high-performance liquid chromatography (RP-HPLC) method using a reversed-phase column (Thermo Scientific Acclaim C30 column) with a simple acetonitrile/water mobile phase. The column is designed to provide high shape selectivity for separation of hydrophobic, structurally related isomers and unique selectivity complementary to other RP columns (e.g., C18). Although another method for analysis of cis and trans isomers of crotamiton by RP-HPLC has been reported, it requires a three-component mobile phase (acetonitrile/phosphate buffer containing 1% triethylamine) to achieve satisfactory results.

The method was developed on our rapid-separation liquid chromatography (RSLC) system (Thermo Scientific Dionex UltiMate 3000 RSLC system).

Have you checked out our Chemistry, Manufacturing, and Control for Drug Development website page for numerous applications and posters on pharma development? If you do not find what you are looking for, do not hesitate to let us know in the Comments box below. Our applications lab will be pleased to consider your request.

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