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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Accelerated Solvent Extraction: Organochlorine Pesticide

  
  
  
  
  
  

sample preparation of organochlorine pesticidesContinuing our series on using Accelerated Solvent Extraction (ASE) which was developed to meet recent and anticipated changes in environmental regulations will cause severe restrictions on the amount of solvent usage in laboratories worldwide in the preparation of solid waste samples.

Here, we present the ASE technique significantly improving Organochlorine Pesticide (OCP) sample extraction methodology! OCPs are manmade chemicals that have been widely used around the world to protect crops, livestock, and homes from damage due to insects. These compounds are also known as Persistent Organic Pollutants (POPs) due to their tendency to resist breakdown in the environment and also are highly stable and insoluble in water and can therefore be absorbed by soils and sediments. Plants in turn can absorb the compounds from the soil, and animals that eat the plants can absorb them―still relatively unchanged. This process is known as bioaccumulation—and it’s happening all the time.  And we’re not immune from this process—the very nature of bioaccumulation is that it concentrates as you move up the food chain. 

While many of these compounds have been banned from use in the United States, many are still manufactured here for use in other countries. The pesticide DDT is perhaps the best known example of an OCP. Banned from use in the 1970’s, DDT and its metabolites are still found in soils and in biological tissue samples of persons who were born well after the ban. Other OCPs include aldrin, dieldrin, and methoxychlor.

Extraction of Chlorinated Pesticides Using Accelerated Solvent Extraction

Application Note 320, Extraction of Chlorinated Pesticides Using Accelerated Solvent Extraction (ASE), (downloadable PDF) describes a simple, fast method for the extraction of chlorinated pesticides from agricultural samples. This method meets the requirements of U.S. EPA Method 3545, which is a high temperature and pressure procedure for extracting water insoluble or slightly water soluble semivolatile organic compounds from soils, clays, sediments, sludges, and waste solids. The EPA Method was developed and validated on an automated extraction system.

The method described in the application note is applicable to the extraction of chlorinated pesticides from soils, clays, wastes, and sediments containing from 5 to 250 μg/kg of the target compounds.

Also, here is a 3.49 min video on the ASE technique and instrument.

Interested in how ASE workflows can improve your productivity for environmental contaminants and help you save time and solvent?  We invite you to write us in the Comments box below.

Comments

The technique seems amazing for several reasons. But I have some questions about its versatility: can I change extraction temperatures and pressures in order to apply it to other pesticides (organophosphates, carbamates), can I extract tissue samples (e.g., roots, leaves) 
Posted @ Thursday, May 03, 2012 10:10 AM by Katia Montenegro
Hi Katia: 
Thank you for visiting our blog and also posting your question. I have forwarded your question to the experts in question so they can respond to you very soon. 
 
Best regards 
Sonya
Posted @ Friday, June 15, 2012 12:23 PM by Sonya Pelia
Dear Katia, 
 
Thank you for your excellent questions! ASE is all about versatility; extraction times, temperatures, pressures, and solvents can all be changed to extract different compounds, including organophosphate pesticides. EPA method 3545 listed above covers changes in temperatures and pressure for several classes of pesticide compounds, and Dionex Application Note 352 provides details on extraction of organophosphorous pesticides, dioxins and furans, PCB’s and more. And ASE can be used for extraction of all types of solid samples, including roots, leaves, grasses, foods, biological tissues, meats, and many more. All that is required is that the sample be homogenous and that a dispersant is used to allow proper extraction. If you’ve some plant tissue, ASE can extract it!  
 
We appreciate your comments, questions, and feedback regarding Accelerated Solvent Extractions. 
 
 
 
Sincerely, 
 
Bill Donovan 
 
MarCom Specialist: Sample Prep and  
 
Chromatography Data Systems 
 
Global Marketing, Chromatography 
 
 
 
Thermo Fisher Scientific 
 
1214 Oakmead Parkway, 
 
Sunnyvale, CA 94085-4261 
 
(408) 481-4261 Office 
 
(408) 735-9413 Fax 
 
bill.donovan@thermofisher.com 
 
Posted @ Friday, June 15, 2012 1:36 PM by Bil Donovan
Hi Katia 
Here is the link to the application note Bill refers to above: 
http://www.dionex.com/en-us/webdocs/40970-AN352-ASE-Persistent-Organic-Pollutants-23Jun2011-LPN1676-03.pdf 
 
Please do not hesitate to let us know if you have more questions. 
 
best regards 
 
sp
Posted @ Friday, June 15, 2012 1:38 PM by Sonya Pelia
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