Charged Aerosol & UV Detection: Analysis of Herbal Medicines
An earlier series of blog posts focused on the HPLC analysis of Chinese traditional medicines (Post 1 and Post II) and generated a lot of interest in our readers. This blog post focuses on three herbal medicines that are extremely popular in many Western countries and our applications demonstrate excellent results in the detection of analytes.
Faster Analysis of Milk Thistle Extracts by UHPLC-UV-CAD
Milk thistle has long been used as an herbal medicine and dietary supplement for promoting digestive health in the West. (This plant is native to the Mediterranean part of Europe and was first described by Dioscorides, a Greek physician in the Roman army, in De Materia Medica.)
Here we present how our HPLC application for the analysis of botanical supplement milk thistle, based on the established Institute for Nutritional Advancement (INA) Method 115.00 for silymarins in milk thistle extracts, was easily converted to UHPLC conditions with our Method Speed-Up Calculator (scroll to below the fold to click the link to the tool). Using the Thermo Scientific Dionex UltiMate 3000 HPLC system, the Thermo Scientific ESA Corona Charged Aerosol Detector (CAD), and our rapid-separation LC column (Thermo Scientific Acclaim RSLC 120 C18 2.2 µm column), the improved method is 5X faster while enhancing resolution. Our UV and CAD detectors complement one another, with the CAD providing nearly universal detection of nonvolatile analytes, similar inter-analyte response independent of chemical structure, and higher sensitivity, wider dynamic range, and more tractable calibration behavior over ELSD. View the results in our D-Library.
UV Absorption Detection of Kavalactones in Kava Kava Extracts
Kavalactones are the active ingredient in the roots of the Kava (or Kava Kava), a shrub commonly found on many of the Pacific Islands. It is considered to have muscle relaxant, anti-convulsive, and anesthetic properties. Kava preparations are sold in the U.S. as capsules or teas.
The application determines kavalactones in Kava Kava extracts using UV absorption detection at 246 nm.
UV Absorption Detection of Phenolic Compounds in Echinacea Extracts
Herbalists consider echinacea one of the best blood purifiers and also an effective antibiotic and it remains one of the top selling herbal remedies in the United States. Constituents of echinacea include polysaccharides, polyacetylenes, sesquiterpenes, polyphenols, and more.
Our application determines phenolic compounds in echinacea extracts using UV absorbance detection at 330 nm and the chromatogram shows separation and detection of several polyphenolic compounds in echinacea extract.
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