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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Glycan Analysis with a Particular Focus on the Immune System

  
  
  
  
  
  
Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

Overcoming Interference Challenges During Trace Elemental Analysis

  
  
  
  
  
  
trace elemental interference in ICP-OES, ICP-MS, ICP-MS analysis

Applications for trace elemental analysis are quite common, ranging from toxic metal analysis in water, lead analysis in toys, to trace metals analysis for semiconductor, with detection level requirements ranging from parts per million (ppm) to sub parts per trillion (ppt). The trace contamination of certain elements can lead to severe health problems or cause defect in products.

ICP-MS Arsenic Speciation in Rice: The Media and The Science

  
  
  
  
  
  
quantification of inorganic arsenic in rice

Recently I watched a very thought provoking documentary by Dispatches on Channel 4 here in the UK which was all about arsenic in rice and rice products (link to U.S. FDA page). As I have a keen interest in ion analysis, I was intrigued to see if the science is consistent with the media story, so took on some research of my own.

Food Testing Using High Resolution LC-MS/MS: A Case Study at RIKILT

  
  
  
  
  
  
lc-ms ms pesticides analysis

Just watched a great 5 minute video which gave an overview on how high performance liquid chromatography (HPLC) coupled to High Resolution Accurate Mass (HRAM) mass spectrometry can help customers challenged with monitoring analytes such as pesticides and veterinary drugs in food and feed samples.

Monoclonal Antibody High-Resolution Separations Using HIC

  
  
  
  
  
  
hic analysis of monoclonal antibodies

A few weeks ago, I featured a method for the analysis of monoclonal antibodies (MAbs) on a recently released hydrophobic interaction chromatography (HIC) column (link to post) and, here, I am pleased to feature another method on another newly released HIC column (Thermo Scientific MAbPac HIC-10 column)! This new column is a high-resolution, silica-based HIC column designed for separation of mAbs and variants, including anti-body conjugates (ADCs) and provides high resolution, excellent bio-compatibility, and selectivity as compared to HIC columns on the market.

Sample Preparation & LC-MS Analysis: Pharma & Nanomaterials in Water

  
  
  
  
  
  
analysis of Nanomaterials in Water

Do you know that nanomaterials (down to 10,000 smaller than the diameter of a human hair) can be natural (viruses, wax crystals on a lotus leaf, spider silk, butterfly wing scales, blood, milk) or manufactured? (link and source Wikipedia.) Manufactured nanoparticles are increasingly being used in pharma drug delivery, disease detection, energy production, and even food and have triggered growing concern regarding their environmental behavior in aquatic environments. The ability to detect and quantify nanomaterials in complex water matrices has become an important issue (link to EU nanomaterials webpage).

GC-MS/MS Workflows for Metabolomics Experiments

  
  
  
  
  
  
gc-ms metabolite analysis

Did you know that there are up to 5 million metabolites (link to Wikipedia) in the plant kingdom? As a result, the identification and monitoring of these compounds can be extremely challenging. Here, I am pleased to present a couple of resources on the use of gas chromatography-mass spectrometry techniques that can increase the number of identifiable and selectively quantifiable metabolites by combining analytical methods for the different phases of the analysis. (By the way, previously I had written about the use of capillary ion chromatography and HR/AM for the metabolite profiling of oral cancers.)

Capillary Ion Chromatography & HR/AM: Metabolite Profiling of Cancer

  
  
  
  
  
  
chromatography analysis of cancer polar metabolites

Did you know that metabolomics (systematic study of the unique chemical fingerprints that specific cellular processes leave behind) is increasingly used in the study of oral cancers? (Definition: Wikipedia)

HPLC Solid-Core Columns For Fast Pharma Analysis (I)

  
  
  
  
  
  
hplc pharma analysis columns

A two-part series on several resources for our solid-core HPLC columns has been on my to-do list for a while now and kept getting pushed back down the list every week until I decided that I simply had to write about them. So, here goes.

Ion Chromatography Speeds Petroleum Refinery Analysis (I)

  
  
  
  
  
  
analysis of amines in oil and gas

I am pleased to feature a 3-part series on our chromatography solutions for the analysis of light, middle, and heavy distillates in petroleum production and scrubber solutions and boiler/cooling water used in petroleum industrial processes. Solutions include our ion chromatography, gas chromatography, liquid chromatography, ICP-OES, GC-ICP-MS, and combustion ion chromatography applications and analytical instruments.

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