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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Scaling Down Sample Extraction for Limited & Volatile Sample Volumes

  
  
  
  
  
  
bioanalysis sample preparation

In an earlier post on the use of our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) for bioanalysis, I covered how these well plates can enhance sample concentration by 20X (link to post), and here I am pleased to present an application note on how these plates can be used to facilitate the scale down of an extraction method for use when sample volume is limited or volatile as in the case of bioanalytical samples.

Sample Preparation for Analytes Susceptible to Degradation or Loss

  
  
  
  
  
  
bioanlysis sample preparation

Here is the third application note in this series on improving sample preparation for bioanalytical and clinical research applications using our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates). The previous two application notes are presented in the following blog posts:

HPLC SPE Well Plates Enhance Sample Concentration by 20X

  
  
  
  
  
  
sample preparation HPLC

Last month, I featured our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) designed specifically for sample preparation for bioanalysis experiments in a blog post, titled, Solid-Phase Extraction (SPE) Micro Elution Plates for Bioanalysis and, here, I am pleased to present a recently application note featuring the use of these well plates.

Solid Phase Extraction (SPE) Micro Elution Plates for Bioanalysis!

  
  
  
  
  
  
Solid Phase Extraction plates for bioanalysis

I am pleased to feature our recently released solid phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction (SPE) Well Plates) designed specifically for bioanalysis experiments. Bioanalysis is moving toward methods that require higher levels of sensitivity and reproducibility with decreasing sample volumes.

SPE & LC-MS/MS Analysis of Cancer Drug Doxorubicin in Human Plasma

  
  
  
  
  
  
hplc analysis of Doxorubicin

Did you know that the cancer drug Doxorubicin is derived from a bacterial species by chemical semisynthesis and is used to treat a wide range of cancers? (Link & source Wikipedia). A pegylated liposomal formulation of doxorubicin is used in cancer treatment and it is essential to establish a fast and robust LC-MS/MS method for separation and determination of liposomal and non-liposomal (free) doxorubicin present in circulating blood.

Automated SPE Speeds GC Analysis of Pesticides in Beverages

  
  
  
  
  
  
GC Analysis of Pesticides in fruit drinks

The poster featured in this blog post has a somewhat funny title which caught my eye when I was reviewing the list of the latest and most popular gas chromatography applications from one of our marketing specialists. The other reason I am writing about this application is because it does seem concerning that a study from Spain (referenced in the poster) found that pesticides in fruit-based soft drinks are 300 times higher than those in tap water (link to study).

SPE and LC-MS/MS Method: Analysis of Vitamin D in Human Plasma

  
  
  
  
  
  
Analysis of Vitamin D metabolites in Human Plasma

After the last post on the use of gas chromatography-mass spectrometry in bioanalysis research studies (link to post), I am pleased to feature a recently released solid-phase extraction (SPE) and LC-MS/MS method for the analysis of the sunshine vitamin, Vitamin D, in human plasma.

HPLC Determination of Growth Stimulants in Farm Animals

  
  
  
  
  
  
fast HPLC analysis of clenbuterol, ractopamine, and phenolethanolamine in meat

This blog post features the efficient and sensitive HPLC analysis of the synthetic compounds clenbuterol, ractopamine, and phenolethanolamine which have been extensively used as growth stimulants in farm animals (and by athletes seeking enhanced performance). The reason for concern is that these compounds, grouped as e β2-agonists, may remain as residues in meat intended for human consumption. While food safety concern for the presence of ractopamine is low, high levels of clenbuterol can cause severely increased heart rate and blood pressure, anxiety, palpitation, and skeletal muscle tremors. (Source & link: Centre for Food Safety in HongKong.) Clenbuterol has been banned by many countries including the USA, European Union, and China; and, the newly introduced phenolethanolamine is already banned in China.

Solid-Phase Extraction: Complete Recovery of Hormones in Water

  
  
  
  
  
  
sample preparation hormones in drinking water

Did you know that the presence of hormones (from both natural and artificial sources) in drinking water is a serious human health concern? This is how they end up in our drinking water supplies: hormones are widely used in pharmaceuticals prescribed to humans and livestock animals, and after consumption, the hormones often end up in the sewage system as a result of excretion and disposal of unwanted quantities of the pharmaceutical drugs. The European Parliament has identified several estrogen variants as priority substances that will be monitored to determine appropriate measures to address the risk posed by these compounds. Several hormones, including estriol, estrone, estradiol, ethynylestradiol, equilin, androstenedione, and testosterone, are routinely monitored as part of the U.S. Environmental Protection Agency (EPA) Unregulated Contaminant Monitoring program (link to webpage). These hormones include estriol, estrone, estradiol, ethynylestradiol, equilin, androstenedione, and testosterone.

Solid-Phase Extraction Speeds Extraction of Surfactants in Wastewater

  
  
  
  
  
  
analysis of surfactants in wastewater

After the last post on the subject of new Solid-Phase Extraction (SPE) cartridges with polymeric phases (link to post), I am pleased to feature a recently developed application for the automated solid-phase extraction of surfactants in wastewater using one of these new cartridges.

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