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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Hydrophobic Interaction Liquid Chromatography: Sorting Through the Hype

Hydrophobic Interaction Liquid Chromatography or HILIC

In my 25 years in the chromatography industry, I have seen tremendous improvements in daily laboratory workflow through the years, in many cases, due to revolutionary products developed to save time and money for the researcher and provide more accurate and reproducible results day after day.  I know how difficult it is to stay current and sort through all the new developments in the industry especially while you’re trying to execute daily activities.  There is little time to explore all the new tools and techniques presented, and it’s hard to know which ones will benefit you. My goal, in this and future blog posts, is to discuss and provide resources on the latest tools and techniques that will help you improve and speed up your day-to-day work in the lab.

Is Your Wine Authentic: An Ion Chromatography Method

authenticating wine

On the agenda of the recent UK Laboratory of the Government Chemist (LGC) meeting I attended was a fascinating talk titled: Wine Fraud – Catching the Cheats (link to downloadable pdf of the talk) by renowned wine expert Geoff Taylor (link to profile) of Campden BRI. Although Geoff’s does not discuss Ion Chromatography (IC), his abstract has some startling facts where fraud could potentially make serious money for the criminals, ranging from the fine wine market where volume is low and unit prices are high through to the mass volume market.

ICP-MS Arsenic Speciation in Rice: The Media and The Science

quantification of inorganic arsenic in rice

Recently I watched a very thought provoking documentary by Dispatches on Channel 4 here in the UK which was all about arsenic in rice and rice products (link to U.S. FDA page). As I have a keen interest in ion analysis, I was intrigued to see if the science is consistent with the media story, so took on some research of my own.

Food Testing Using High Resolution LC-MS/MS: A Case Study at RIKILT

lc-ms ms pesticides analysis

Just watched a great 5 minute video which gave an overview on how high performance liquid chromatography (HPLC) coupled to High Resolution Accurate Mass (HRAM) mass spectrometry can help customers challenged with monitoring analytes such as pesticides and veterinary drugs in food and feed samples.

Pesticides Analysis with High-Resolution Accurate Mass Spectrometry

high resolution mass spectrometry, HRAM, high resolution accurate mass, pesticide, veterinary drug, orbitrap, LC-MS, LC-MS/MS

Liquid chromatography with triple quadrupole mass spectrometry (LC-MS/MS) is considered the gold standard for screening and quantitation of pesticides in various food matrices. However, high-resolution accurate mass (HRAM) LC-MS/MS is growing in popularity, in part because it allows for retrospective analysis on additional analytes of interest and screening for unknown or unexpected pesticides is possible. This is because all ions can be collected and fragmented whereas triple quadrupole mass spectrometry requires a target list of compounds. Furthermore, method development time is often shorter by orders of magnitude with HRAM LC-MS/MS, enabling the screening of a large number of compounds compared to triple quadrupole mass spectrometers.

Pomegranate Juice Fraud: HPLC Detects What's in Your Juice!

Pomegranate Juice adulteration testing

Did you know that a peer-reviewed study, titled, International multidimensional authenticity specification (IMAS) algorithm for detection of commercial pomegranate juice adulteration, (link to study), published in the J Agric Food Chem journal, found that only 35% of the commercially available pomegranate juice qualifies as authentic pomegranate juice?

GC-MS/MS Improves Detection of Low-Level Dioxins in Food & Feed

analysis of low levels of dioxins in food

Did you know that more than 90% of human exposure to highly toxic dioxins is through food, mainly meat and dairy products, fish and shellfish? (Link & source WHO page on dioxins.) A little about dioxins in case you are not familiar with these chemicals. Dioxins are a group of chemically-related compounds, including polychlorinated dibenzodioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and some dioxin-like polychlorinated biphenyls (PCBs) which are formed by waste incineation, forest fires, and backyard trash burning. These compounds are classified as persistent organic pollutants (POPs) as they are highly toxic and bioaccumulate in food chains, mainly in the fatty tissues of animals. Exposure to dioxins is known to cause developmental problems, damage to the immune system, dysregulation of hormone levels and cancer.

HPLC Simplifies Analysis of Artificial Sweetner Acesulfame in Vinegar

analysis of acesulfame potassium by hplc

I found the HPLC application, briefly discussed in this blog post, for the analysis of a synthetic sweetener called Acesulfame or Acesulfame K in vinegar fascinating as I had no idea that it is extensively used as a sugar substitute in foods and beverages. Some food regulatory bodies, such as The Standardization Administration of China allows Acesulfame to be added to fermented vinegar products, but limits the added amount to no more than 4 µg/mL. Therefore, an efficient method is needed to sensitively and rapidly determine Acesulfame in fermented vinegar. By the way, Acesulfame is 200 times sweeter than sucrose.

Gas Chromatography Analysis of Methane & Carbon Dioxide in Beer Waste

analysis of spent brewers grain

Did you know that the world beer consumption was 187.37 million kiloliters in 2012  and China remains the largest beer-consuming country for the 10th year running? (Source and link to Kirin Beer University Report.) The challenge remains of dealing the spent brewer's grain used to produce beer, and typically, over 300,000 tonnes of this spent grain is produced annually. While it is used successfully as an animal feedstock, the spent grain deteriorates rapidly due to its high humidly and fermenting sugar content. In the gas chromatography research study, presented in this post, researchers present novel methods for drying the spent grain by using the bio-energy generated from the anaerobic digestion of the spent grain in the same brewery!

HPAE-PAD Speeds Analysis of Carbohydrates in Beverages & Milk

analysis of carbohydrates in apple cider

The ongoing challenge for food regulatory bodies is not just identifying food contamination problems but also making sure that product formulation and product quality meets regulatory standards and food manufacturers report any ingredients that might impact immune-and allergy-sensitive individuals. By the way, did you know that India has banned the import of milk and milk products from China until June 2015 due to concerns of contamination of melamine? And. you can read about our solutions on the analysis of melamine in pet food and milk (link to post) on this blog.

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