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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Troubleshooting Pharmaceutical Assays in HPLC & UHPLC

troubleshooting pharmaceutical HPLC and UHPLC applications

The focus of this post to present several resources for troubleshooting UHPLC and HPLC methods in pharmaceutical and biopharmaceutical applications. How this post came about was with my receiving the first resource via an email and then learning about the webinar.

Glycan Analysis with a Particular Focus on the Immune System

Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

Isomeric Separation & Structural Identification of Labeled N-linked Glycans

HILIC glycan analysis

Analysis of glycosylation is a topic of growing importance in a number of fields such as antibody and biopharmaceutical characterization and human glycoproteome characterization for disease biomarkers. Variations in glycosylation have been implicated in a number of conditions including autoimmune disease, cancer and infectious disease as well as the correct functioning of the immune system, more details of which can be found in the review article by Zauner et al entitled, Glycoproteomic Analysis of Antibodies, (link to article).

Fast Pharma Drug (Nevirapine) Impurity Profiling Using UHPLC-DAD

Pharma Drug Nevirapine Impurity Profiling

It has been a long while, almost two months, since I wrote about pharmaceutical drug analysis on this blog in post, titled, Charged Aerosol Detection Speeds Analysis of Pharma Drug Impurities (link to post), and I found the UHPLC application featured here while looking for something else on our website. I am pleased to write about this application because it features our latest UHPLC system (Thermo Scientific Vanquish UHPLC system) and can be used with our method transfer tool to speed and streamline the process of adapting HPLC parameters for UHPLC analysis.

UHPLC Techniques Speed Analysis of Herbicides

herbicide analysis hplc

I recently received an email notifying me of a newly released herbicide method featuring the use of our latest UHPLC system (Thermo Scientific Vanquish UHPLC system) and one of our solid-core UHPLC columns (Thermo Scientific Accucore Vanquish C18 1.5 μm UHPLC column), and realized it has been a quite sometime since I shared an herbicide application on this blog. So, here goes!

UHPLC-MS Analysis of Psychotropics in Mushrooms and Chocolate

Psychotropics in Mushrooms and Chocolate

I found this fascinating application, featured here, presenting an UHPLC-MS method for the analysis of the psychotropic substances psilocybin and psilocin in mushrooms and chocolate on our website while looking for information on one of our UHPLC systems and thought it would make a great read on this blog! The method was developed by our chemists at our San Jose lab (California, USA) in collaboration with the Crime Laboratory of the Washington State Patrol (Washington, USA) to aid in the identification of controlled substances, such as psychotropics, in small quantities of seized evidence.

Chromatography and Sample Preparation Terminology Guide

chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

HPLC Applications Notebook: Traditional Chinese Medicine

Traditional Chinese Medicine HPLC analysis

Did you know that the oldest work on Traditional Chinese Medicine (TCM) appeared in 200 B.C. and that the first TCMs and acupuncture needles appeared around 2500 BCE in China? (Link to site.) And, here I am pleased to present to you our recently released notebook featuring HPLC applications for TCM to help ensure the efficacy, purity and safety of these traditional remedies!

Electrochemical Detection Troubleshooting

troubleshooting Electrochemical Detection (ECD)

I came across two technical notes on troubleshooting Electrochemical Detection (ECD) problems, featured in this blog post, while reading up on a recently released HPLC application that used this type of detection. And, soon after, I found a poster note on the topic and thought this would make for a useful read for those working in HPLC-ECD applications. For those new to this type of detection, ED is the most sensitive and selective mode of LC detection for the measurement of oxidizable or reducible compounds.

Monoclonal Antibody Characterization: Technical Guide on Columns

hplc Monoclonal Antibody Characterization

I am pleased to present here our recently released Biomolecules Technical Guide (downloadable PDF) on the HPLC column best suited to Monoclonal Antibody (mAb) characterization. If you work in this field, you will be aware that mAb treatment is one of the most effective methods of diagnosis and treatment of a broad range of diseases, including autoimmune disorders, cardiovascular diseases, infectious diseases, cancer, and inflammation. And, since there is an extensive development pipeline for protein and mAb therapeutics, there is a need for innovative and efficient analytic tools.

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