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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Food Testing Using High Resolution LC-MS/MS: A Case Study at RIKILT

lc-ms ms pesticides analysis

Just watched a great 5 minute video which gave an overview on how high performance liquid chromatography (HPLC) coupled to High Resolution Accurate Mass (HRAM) mass spectrometry can help customers challenged with monitoring analytes such as pesticides and veterinary drugs in food and feed samples.

Pesticides Analysis with High-Resolution Accurate Mass Spectrometry

high resolution mass spectrometry, HRAM, high resolution accurate mass, pesticide, veterinary drug, orbitrap, LC-MS, LC-MS/MS

Liquid chromatography with triple quadrupole mass spectrometry (LC-MS/MS) is considered the gold standard for screening and quantitation of pesticides in various food matrices. However, high-resolution accurate mass (HRAM) LC-MS/MS is growing in popularity, in part because it allows for retrospective analysis on additional analytes of interest and screening for unknown or unexpected pesticides is possible. This is because all ions can be collected and fragmented whereas triple quadrupole mass spectrometry requires a target list of compounds. Furthermore, method development time is often shorter by orders of magnitude with HRAM LC-MS/MS, enabling the screening of a large number of compounds compared to triple quadrupole mass spectrometers.

HPLC Toxiology Research Articles on Analysis of Illicit Drugs

hplc toxicology analysis

I have mentioned before on this blog that I am fascinated by the subject of toxicology and find peer-reviewed articles featuring the use of chromatography, such as the two articles featured here, absolutely riveting. By the way, did you know that the pace at which new and illicit psychoactive substances have been emerged has speeded up tremendously in the last few years?

Chromatography and Sample Preparation Terminology Guide

chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

Scaling Down Sample Extraction for Limited & Volatile Sample Volumes

bioanalysis sample preparation

In an earlier post on the use of our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) for bioanalysis, I covered how these well plates can enhance sample concentration by 20X (link to post), and here I am pleased to present an application note on how these plates can be used to facilitate the scale down of an extraction method for use when sample volume is limited or volatile as in the case of bioanalytical samples.

Sample Preparation & LC-MS Analysis: Pharma & Nanomaterials in Water

analysis of Nanomaterials in Water

Do you know that nanomaterials (down to 10,000 smaller than the diameter of a human hair) can be natural (viruses, wax crystals on a lotus leaf, spider silk, butterfly wing scales, blood, milk) or manufactured? (link and source Wikipedia.) Manufactured nanoparticles are increasingly being used in pharma drug delivery, disease detection, energy production, and even food and have triggered growing concern regarding their environmental behavior in aquatic environments. The ability to detect and quantify nanomaterials in complex water matrices has become an important issue (link to EU nanomaterials webpage).

Sample Preparation for Analytes Susceptible to Degradation or Loss

bioanlysis sample preparation

Here is the third application note in this series on improving sample preparation for bioanalytical and clinical research applications using our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates). The previous two application notes are presented in the following blog posts:

LC-MS Platform for the Analysis of Finished Petroleum Products (II)

chemical characterization of petroleum

In Part II of this series on chromatography solutions for the analysis of oil and gas refinery products, I am pleased to present a robust LC-MS method for the complete analysis of finished petroleum products. (Part I is featured here: Ion Chromatography Speeds Petroleum Refinery Analysis (I).)

LC-MS Solutions for Analysis of Marine Biotoxins in Shellfish

hplc analysis of marine biotoxins in shellfish

Did you know that marine biotoxins resulting from algal blooms can accumulate in bivalve mollusks as these organisms filter large quantities of water and can poison human beings who consume the mollusks? For example, in the State of New York (USA), one can check the sanitary conditions of shellfish lands and find out if they are closed for shellfish collection (link to site). Studies have shown that phosphorus in agricultural fertilizer is the primary cause of toxic algal blooms, as in the Great Lakes in the USA (link to article).

HPLC SPE Well Plates Enhance Sample Concentration by 20X

sample preparation HPLC

Last month, I featured our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) designed specifically for sample preparation for bioanalysis experiments in a blog post, titled, Solid-Phase Extraction (SPE) Micro Elution Plates for Bioanalysis and, here, I am pleased to present a recently application note featuring the use of these well plates.

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