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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Scaling Down Sample Extraction for Limited & Volatile Sample Volumes

bioanalysis sample preparation

In an earlier post on the use of our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) for bioanalysis, I covered how these well plates can enhance sample concentration by 20X (link to post), and here I am pleased to present an application note on how these plates can be used to facilitate the scale down of an extraction method for use when sample volume is limited or volatile as in the case of bioanalytical samples.

Sample Preparation & LC-MS Analysis: Pharma & Nanomaterials in Water

analysis of Nanomaterials in Water

Do you know that nanomaterials (down to 10,000 smaller than the diameter of a human hair) can be natural (viruses, wax crystals on a lotus leaf, spider silk, butterfly wing scales, blood, milk) or manufactured? (link and source Wikipedia.) Manufactured nanoparticles are increasingly being used in pharma drug delivery, disease detection, energy production, and even food and have triggered growing concern regarding their environmental behavior in aquatic environments. The ability to detect and quantify nanomaterials in complex water matrices has become an important issue (link to EU nanomaterials webpage).

Sample Preparation for Analytes Susceptible to Degradation or Loss

bioanlysis sample preparation

Here is the third application note in this series on improving sample preparation for bioanalytical and clinical research applications using our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates). The previous two application notes are presented in the following blog posts:

LC-MS Platform for the Analysis of Finished Petroleum Products (II)

chemical characterization of petroleum

In Part II of this series on chromatography solutions for the analysis of oil and gas refinery products, I am pleased to present a robust LC-MS method for the complete analysis of finished petroleum products. (Part I is featured here: Ion Chromatography Speeds Petroleum Refinery Analysis (I).)

LC-MS Solutions for Analysis of Marine Biotoxins in Shellfish

hplc analysis of marine biotoxins in shellfish

Did you know that marine biotoxins resulting from algal blooms can accumulate in bivalve mollusks as these organisms filter large quantities of water and can poison human beings who consume the mollusks? For example, in the State of New York (USA), one can check the sanitary conditions of shellfish lands and find out if they are closed for shellfish collection (link to site). Studies have shown that phosphorus in agricultural fertilizer is the primary cause of toxic algal blooms, as in the Great Lakes in the USA (link to article).

HPLC SPE Well Plates Enhance Sample Concentration by 20X

sample preparation HPLC

Last month, I featured our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) designed specifically for sample preparation for bioanalysis experiments in a blog post, titled, Solid-Phase Extraction (SPE) Micro Elution Plates for Bioanalysis and, here, I am pleased to present a recently application note featuring the use of these well plates.

Charged Aerosol Detection of Surfactants in mAb Formulation

Surfactant analysis in Monoclonal Antibody Formulation

This post briefly presents a very recently released research study featuring the use of our Charged Aerosol Detector (CAD) and two elegant UHPLC-CAD-MS methods for the analysis of polysorbate 20 in monoclonal antibody (mAb) formulation. (Image is of an Immunoglobulin G, IgG1, mAb molecule.)

Challenges of Intact Protein LC-MS Work: On-Demand Webinar

intact protein separations

I just realized that it has been a long time since I wrote about a proteomics application on this blog and the on-demand LC-MS webinar featured here fits the bill! The goal of the webinar is that by the end of the webinar, the participant will be able to perform foolproof intact protein analysis, regardless of their expertise in this area.

SPE & LC-MS/MS Analysis of Cancer Drug Doxorubicin in Human Plasma

hplc analysis of Doxorubicin

Did you know that the cancer drug Doxorubicin is derived from a bacterial species by chemical semisynthesis and is used to treat a wide range of cancers? (Link & source Wikipedia). A pegylated liposomal formulation of doxorubicin is used in cancer treatment and it is essential to establish a fast and robust LC-MS/MS method for separation and determination of liposomal and non-liposomal (free) doxorubicin present in circulating blood.

SPE and LC-MS/MS Method: Analysis of Vitamin D in Human Plasma

Analysis of Vitamin D metabolites in Human Plasma

After the last post on the use of gas chromatography-mass spectrometry in bioanalysis research studies (link to post), I am pleased to feature a recently released solid-phase extraction (SPE) and LC-MS/MS method for the analysis of the sunshine vitamin, Vitamin D, in human plasma.

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