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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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HPLC Method for Determination of Benzo(a)pyrene in Meat Products

  
  
  
  
  
  
Benzo(a)pyrene in smoked meat

Here, I am pleased to present an efficient HPLC method featuring an improved sample preparation method with fluorescence detection for the sensitive and rapid determination of benzo[a]pyrene (downloadable U.S. EPA factsheet) in complex meat products, such as sausages and preserved ham. 

HPLC Speeds Analysis of Rhodamine B in Cosmetics

  
  
  
  
  
  
analysis of cosmetics

I was fascinated by the HPLC application described in this post as it deals with chemicals, in particular Rhodamine B, (link to Wikipedia page), in cosmetics, and I am always curious to know what could be lurking in cosmetics that I might be using. Many regulatory bodies around the globe, such as the U.S. FDA (link to cosmetic labeling guide), European Commission (link to EU regulations on cosmetics), India's Bureau of Standards (link to regulations for cosmetics), and China's Food and Drug Administration (link to cosmetics regulatory pages) regulate the ingredients, testing, and labeling of cosmetics.

HPLC & CAD: Separation of Biochemical Buffering Agents

  
  
  
  
  
  
analysis of buffering agents

Good’s buffers are a series of 20 zwitterionic buffering agents that are used in biochemical experiments and HPLC mobile phases and feature stable pH control, low biological activity, low UV absorbance, and low metal affinity. This post features a newly released method separating 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions.

Hydrophobic Interaction Chromatography Method for MAb Analysis

  
  
  
  
  
  
analysis of papain digests

I am pleased to feature one of our hydrophobic interaction chromatography (HIC) columns (Thermo Scientific ProPac HIC-10 column) in a newly released method describing the separation of low-concentration species of an intentionally oxidized monoclonal antibody (MAb) from the native MAb. Those working in the field of developing MAbs as human therapeutics are fully aware of the challenges of working with MAbs, which can include various post-translational modifications and degradation during storage and delivery.

HPLC Speeds Determination of Dicyandiamide in Milk Powder

  
  
  
  
  
  
dicyandiamide analysis in milk powder

Here, I am pleased to feature an efficient and simple reliable HPLC method for the monitoring of dicyandiamide levels in milk and milk-derived products; last year, a New Zealand company found traces of this toxic chemical in its milk products (link to story).

HPLC Solution Kit for Simultaneous Analysis of Pharma Counterions

  
  
  
  
  
  
hplc counterion analysis

I am pleased to present our recently released HPLC counterion solution kit for pharmaceutical QA/QC (Thermo Scientific IonCount solution) which simplifies the analysis and setup of complete HPLC workflows for common and less common organic and inorganic counterions in pharmaceutical analysis. Those working in this field know that obtaining qualitative and quantitative information of pharmaceutical drugs and residual salts is critical for determining the purity, mass balance and stoichiometry of drug formulations and this solution can speed up the screening and assaying of a broad variety of counterions and active pharmaceutical ingredients (APIs).

Chromatography and Sample Preparation Terminology Guide

  
  
  
  
  
  
chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

HPLC Method for Metabolite Profiling of Wine, Juice, & Tea

  
  
  
  
  
  
metabolite profiling of tea wine fruit juice

I am pleased to present a recently released application note featuring a gradient HPLC method to study product authenticity, such as differentiating wine varietals, orange juice varietals and growing region, and distinguishing tea blends, and identifying adulteration in juices, such the blending of orange juice with cheaper juices or the inclusion of peel or pulp wash.

HPLC & CAD: Surfactants and Emulsifiers Applications Notebook

  
  
  
  
  
  
analysis of emulsifiers and surfactants using hplc

Did you know that the global food emulsifiers market is one of the fastest growing markets in the food ingredient market and is estimated to grow to $2,858.6 million by 2018? (Link to market research website.) Emulsifiers are used to stabilize emulsions in the food industry plus used as an additive to reduce fat content. Surfactants are compounds that lower the surface tension between liquids or a liquid and a solid, and are used as detergents, wetting agents, emulsifiers, foaming agents, and dispersants. (Definition and link Wikipedia page.) Other important uses of surfactants include industrial applications in lubrication, textile processing, petroleum recovery, and wastewater treatment.

HPLC Applications Notebook: Traditional Chinese Medicine

  
  
  
  
  
  
Traditional Chinese Medicine HPLC analysis

Did you know that the oldest work on Traditional Chinese Medicine (TCM) appeared in 200 B.C. and that the first TCMs and acupuncture needles appeared around 2500 BCE in China? (Link to site.) And, here I am pleased to present to you our recently released notebook featuring HPLC applications for TCM to help ensure the efficacy, purity and safety of these traditional remedies!

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