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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Why I Turned from Phosphorylation to Glycan Analysis

  
  
  
  
  
  
glycosylation analysis by UHPLC

Most of my early research career focused on the post-translational modifications (PTMs) of proteins and in those days there was only one worthy PTM in my eyes and that was phosphorylation while glycosylation hardly registered. That was because phosphorylation governed important cellular processes (in my view at least) such as cell signalling; however, my love of phosphorylation slowly waned as I grew to understand the only reliable method to measure phosphorylation was with the potentially harmful radioactive Phosphorus-32 (32P)! (Link to Wikipedia page.) That’s when I started to get more interested in glycosylation and the huge diversity of modifications this could entail, after all phosphorylation was only adding phosphate on to a serine, threonine or tyrosine. Glycosylation offered more possibilities and intrigue and the opportunity to use other analytical techniques that didn’t involve 32P.

Give Your Valentine: Antioxidants!

  
  
  
  
  
  
analysis of polyphenols in wine

Of all the special days on the calendar, I think Valentine’s Day has to be the healthiest. Think about it. Christmas has all those fattening cookies, fruitcake, and candy canes – not to mention the stress of shopping, wrapping, and paying off the credit cards. Thanksgiving is known for overeating and has pies, pies, pies. Halloween? Please. The Fourth of July is grilled meat (HCAs and PAHs anyone?) and ice cream. But Valentine’s Day is red wine, chocolate, and flowers.

Authenticity of Dietary Supplements in Question: A Recall in New York

  
  
  
  
  
  
dietary supplement authentication by HPLC

The authenticity and efficacy of dietary supplements (DS) have been a concern for many years now. And, on Tuesday, February 3, the New York State Attorney General’s Office announced a state-wide recall of herbal supplements sold through multiple major retailers (link to story). The recall is a result of a study conducted by authorities that found that many of these products were fraudulent and potentially dangerous. Supplements called into question include products said to contain Ginkgo Biloba and St. John’s wort, amongst others.

A Milestone in Pesticides Analysis for Orbital Trap Mass Spectrometry

  
  
  
  
  
  

Australia Recalls Tonic Water – The Hazards of Food Mislabeling

  
  
  
  
  
  
analysis of quinine in tonic water

Earlier this month I read that Australia has recalled Indian Tonic Water (link to story) as some of the tonic water bottles were mislabeled as soda water and, hence, the labels did not include information on presence of quinine in the beverages; both Australia and the EU require that the presence of quinine in such beverages be declared. Indeed, the UK's SCHEDULE 8: MISLEADING DESCRIPTIONS states that "The name “Indian tonic water” or “quinine tonic water” shall not be applied to any drink unless the drink contains not less than 57 mg of quinine (calculated as quinine sulphate B.P.) per litre of the drink.

Sample Preparation: Three Techniques Reviewed

  
  
  
  
  
  
hplc sample preparation

I hope you found the last installment on Hydrophobic Interaction Liquid Chromatography (link to blog post) useful. Supporting my goal to discuss and provide resources on the latest tools and techniques that will help improve and speed up your day-to-day work in the lab, I will discuss sample preparation for HPLC in this post.

Troubleshooting Pharmaceutical Assays in HPLC & UHPLC

  
  
  
  
  
  
troubleshooting pharmaceutical HPLC and UHPLC applications

The focus of this post to present several resources for troubleshooting UHPLC and HPLC methods in pharmaceutical and biopharmaceutical applications. How this post came about was with my receiving the first resource via an email and then learning about the webinar.

Glycan Analysis with a Particular Focus on the Immune System

  
  
  
  
  
  
Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

Is Your Wine Authentic: An Ion Chromatography Method

  
  
  
  
  
  
authenticating wine

On the agenda of the recent UK Laboratory of the Government Chemist (LGC) meeting I attended was a fascinating talk titled: Wine Fraud – Catching the Cheats (link to downloadable pdf of the talk) by renowned wine expert Geoff Taylor (link to profile) of Campden BRI. Although Geoff’s does not discuss Ion Chromatography (IC), his abstract has some startling facts where fraud could potentially make serious money for the criminals, ranging from the fine wine market where volume is low and unit prices are high through to the mass volume market.

HPLC Conference Overload & Fractal Chromatography Columns

  
  
  
  
  
  
hplc protein peptide analysis

I occasionally get conference overload, and this was the case a few months ago with three chromatography conferences in two weeks (HPLC 2014, ISCC at Riva, and the UK ChromSoc AGM), two oral presentations and five posters to present plus the many seminars given by other delegates to listen to as well. It sounds busy and it is intensive as most of the hard work comes from the interactions outside of the lecture halls.

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