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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Is Your Wine Authentic: An Ion Chromatography Method

  
  
  
  
  
  
authenticating wine

On the agenda of the recent UK Laboratory of the Government Chemist (LGC) meeting I attended was a fascinating talk titled: Wine Fraud – Catching the Cheats (link to downloadable pdf of the talk) by renowned wine expert Geoff Taylor (link to profile) of Campden BRI. Although Geoff’s does not discuss Ion Chromatography (IC), his abstract has some startling facts where fraud could potentially make serious money for the criminals, ranging from the fine wine market where volume is low and unit prices are high through to the mass volume market.

HPLC Conference Overload & Fractal Chromatography Columns

  
  
  
  
  
  
hplc protein peptide analysis

I occasionally get conference overload, and this was the case a few months ago with three chromatography conferences in two weeks (HPLC 2014, ISCC at Riva, and the UK ChromSoc AGM), two oral presentations and five posters to present plus the many seminars given by other delegates to listen to as well. It sounds busy and it is intensive as most of the hard work comes from the interactions outside of the lecture halls.

Fast Pharma Drug (Nevirapine) Impurity Profiling Using UHPLC-DAD

  
  
  
  
  
  
Pharma Drug Nevirapine Impurity Profiling

It has been a long while, almost two months, since I wrote about pharmaceutical drug analysis on this blog in post, titled, Charged Aerosol Detection Speeds Analysis of Pharma Drug Impurities (link to post), and I found the UHPLC application featured here while looking for something else on our website. I am pleased to write about this application because it features our latest UHPLC system (Thermo Scientific Vanquish UHPLC system) and can be used with our method transfer tool to speed and streamline the process of adapting HPLC parameters for UHPLC analysis.

Charged Aerosol Detection Speeds Analysis of Botanicals

  
  
  
  
  
  
tcm analysis

Previously, this blog featured liquid chromatography analysis of Traditional Chinese Medicine (TCM) in a blog post that presented a HPLC applications notebook (link to blog post) on the subject; here, I discuss an HPLC application for the analysis of botanicals and natural products.

Pomegranate Juice Fraud: HPLC Detects What's in Your Juice!

  
  
  
  
  
  
Pomegranate Juice adulteration testing

Did you know that a peer-reviewed study, titled, International multidimensional authenticity specification (IMAS) algorithm for detection of commercial pomegranate juice adulteration, (link to study), published in the J Agric Food Chem journal, found that only 35% of the commercially available pomegranate juice qualifies as authentic pomegranate juice?

Charged Aerosol Detection vs. Evaporative Light Scattering Detection

  
  
  
  
  
  
Charged Aerosol Detection

I recently received a notification on a recently released poster note (presented at the 2014 AOAC Conference) exploring the fundamental differences between Charged Aerosol Detection (CAD) and Evaporative Light Scattering Detection (ELSD) (link to article) and thought it would make an excellent read for those chromatographers wishing to learn more about these two detection techniques.

HPLC Improves Analytical Method for Protein Quality Control in MAbs

  
  
  
  
  
  
monoclonal antibody analysis

After the last post on the topic of HPLC analyis of monoclonal antibodies (MAbs) using a newly released  hydrophobic interaction chromatography (HIC) column (Thermo Scientific MAbPac HIC-10 column), I am pleased to present another recently released application note featuring a pH-gradient-based method that provides excellent resolution for MAb charge variants.

HPLC Measurement of Copper Additives in Electroplating Baths

  
  
  
  
  
  
Copper Additives in Electroplating Baths

I know the HPLC analysis of plating baths for electronics may not sound exciting but I found the white paper featured here on this subject to be fascinating reading. And, it led me to doing a little research. Did you know that damascene, or acid copper plating baths, have been in use since the mid 19th century on decorative items and machinery, including integrated circuits and satellites in recent times? And, that the art of inlaying different metals into one another is called damascening, from the resemblance of such items to patterns of damask silk (link & source Wikipedia)? 

HPLC Simplifies Analysis of Artificial Sweetner Acesulfame in Vinegar

  
  
  
  
  
  
analysis of acesulfame potassium by hplc

I found the HPLC application, briefly discussed in this blog post, for the analysis of a synthetic sweetener called Acesulfame or Acesulfame K in vinegar fascinating as I had no idea that it is extensively used as a sugar substitute in foods and beverages. Some food regulatory bodies, such as The Standardization Administration of China allows Acesulfame to be added to fermented vinegar products, but limits the added amount to no more than 4 µg/mL. Therefore, an efficient method is needed to sensitively and rapidly determine Acesulfame in fermented vinegar. By the way, Acesulfame is 200 times sweeter than sucrose.

Monoclonal Antibody High-Resolution Separations Using HIC

  
  
  
  
  
  
hic analysis of monoclonal antibodies

A few weeks ago, I featured a method for the analysis of monoclonal antibodies (MAbs) on a recently released hydrophobic interaction chromatography (HIC) column (link to post) and, here, I am pleased to feature another method on another newly released HIC column (Thermo Scientific MAbPac HIC-10 column)! This new column is a high-resolution, silica-based HIC column designed for separation of mAbs and variants, including anti-body conjugates (ADCs) and provides high resolution, excellent bio-compatibility, and selectivity as compared to HIC columns on the market.

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