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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Electrochemical Detection Troubleshooting

  
  
  
  
  
  
troubleshooting Electrochemical Detection (ECD)

I came across two technical notes on troubleshooting Electrochemical Detection (ECD) problems, featured in this blog post, while reading up on a recently released HPLC application that used this type of detection. And, soon after, I found a poster note on the topic and thought this would make for a useful read for those working in HPLC-ECD applications. For those new to this type of detection, ED is the most sensitive and selective mode of LC detection for the measurement of oxidizable or reducible compounds.

Scaling Down Sample Extraction for Limited & Volatile Sample Volumes

  
  
  
  
  
  
bioanalysis sample preparation

In an earlier post on the use of our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates) for bioanalysis, I covered how these well plates can enhance sample concentration by 20X (link to post), and here I am pleased to present an application note on how these plates can be used to facilitate the scale down of an extraction method for use when sample volume is limited or volatile as in the case of bioanalytical samples.

Chromatography Analysis of Additives & More in Beverages (II)

  
  
  
  
  
  
analysis of additives in beverage

After last month's post featuring chromatography applications for the analysis of sugars and artificial sweeteners in beverages (link to post), I am pleased to present two more application notebooks for the analysis of beverages, one for the analysis of carbonated beverages and the other for the analysis of additives in beverages.

Creating An HPLC Affinity Titer Column for Monoclonal Antibody Analysis

  
  
  
  
  
  
monoclonal antibody analysis

In the poster presented in this blog post, scientists from our Sunnyvale (USA) research lab describe a straightforward method for creating designer assays for QC analysis of monoclonal antibodies (mAb) using our HPLC-compatible, streptavidin-coated monolith column. This affinity column produces narrow concentrated peaks with minimal diffusion, allows for titer analysis over a wide concentration range, and is applicable for a range of applications from small-scale protein to antibody titer analysis.

Chromatography Analysis of Sugars & Sweeteners in Beverages (I)

  
  
  
  
  
  
chromatography sugars in beverages

Have you been following the increasing efforts by various governments to regulate the amount of sugar in beverages or impose sugar taxes on sugary drinks in an effort to reduce sugar in food and beverage products? In one story, UK's chief medical officer told a committee of MPs that a sugar tax may have to be introduced to curb obesity rates (link to story) and in another story, the state of Illinois, in the USA, is considering a new tax on sugary beverages (link to story). By the way, a new study concluded that fruit juice has only a bit less fructose than sugary sodas (link to study).

HPLC Solid-Core Columns For Fast Pharma Analysis (II)

  
  
  
  
  
  
HPLC analysis of Analgesic Compounds

In Part II on our solid-core HPLC columns, I am pleased to feature two application notes featuring the use of these columns in pharma and biopharma development. (Part I, HPLC Solid-Core Columns For Fast Pharma Analysis (I), featured a technical article, a technical note, and an on-demand webinar.)

Sample Preparation for Analytes Susceptible to Degradation or Loss

  
  
  
  
  
  
bioanlysis sample preparation

Here is the third application note in this series on improving sample preparation for bioanalytical and clinical research applications using our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLAµ Solid Phase Extraction [SPE] Well Plates). The previous two application notes are presented in the following blog posts:

Chromatography Solutions for Analysis of Milk

  
  
  
  
  
  
hplc milk analysis
How this blog post on the chromatography analysis of milk and milk products came about was the featuring of the following two fascinating stories on our twitter stream and our Chromatography Solutions Facebook page on the topic:
  • Did you know that before refrigeration, people in Russia and Finland dropped live brown frogs into milk to keep it fresh? Moscow State University researchers discovered the science behind this odd custom; they found that amphibians' skin secretions are filled with peptides, which are antimicrobial compounds that are as effective against Salmonella and Staphylococcus bacteria as prescription antibiotics. 
  • The ability of geographically diverse Africans to digest milk has been linked to spread of cattle raising. Researchers found that the ability to digest milk became a selective force in the populations that raised cattle and consumed fresh cow, camel, or goat milk and identified three additional genetic variants with lactase persistence that had not been identified previously. By the way, it was the largest ever study of its kind in Africa.

In our 90-plus page Beverages Applications Notebook: Milk (downloadable PDF), we present applications for monitoring the compositional quality and safety of milk and milk products such as baby formula, lactose-free milk products, and milk for tracking contamination, adulteration, product consistency, and to ensure regulatory compliance.

HPLC Solid-Core Columns For Fast Pharma Analysis (I)

  
  
  
  
  
  
hplc pharma analysis columns

A two-part series on several resources for our solid-core HPLC columns has been on my to-do list for a while now and kept getting pushed back down the list every week until I decided that I simply had to write about them. So, here goes.

LC-MS Platform for the Analysis of Finished Petroleum Products (II)

  
  
  
  
  
  
chemical characterization of petroleum

In Part II of this series on chromatography solutions for the analysis of oil and gas refinery products, I am pleased to present a robust LC-MS method for the complete analysis of finished petroleum products. (Part I is featured here: Ion Chromatography Speeds Petroleum Refinery Analysis (I).)

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