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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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HPLC Simplifies Analysis of Artificial Sweetner Acesulfame in Vinegar

analysis of acesulfame potassium by hplc

I found the HPLC application, briefly discussed in this blog post, for the analysis of a synthetic sweetener called Acesulfame or Acesulfame K in vinegar fascinating as I had no idea that it is extensively used as a sugar substitute in foods and beverages. Some food regulatory bodies, such as The Standardization Administration of China allows Acesulfame to be added to fermented vinegar products, but limits the added amount to no more than 4 µg/mL. Therefore, an efficient method is needed to sensitively and rapidly determine Acesulfame in fermented vinegar. By the way, Acesulfame is 200 times sweeter than sucrose.

Monoclonal Antibody High-Resolution Separations Using HIC

hic analysis of monoclonal antibodies

A few weeks ago, I featured a method for the analysis of monoclonal antibodies (MAbs) on a recently released hydrophobic interaction chromatography (HIC) column (link to post) and, here, I am pleased to feature another method on another newly released HIC column (Thermo Scientific MAbPac HIC-10 column)! This new column is a high-resolution, silica-based HIC column designed for separation of mAbs and variants, including anti-body conjugates (ADCs) and provides high resolution, excellent bio-compatibility, and selectivity as compared to HIC columns on the market.

HPLC Toxiology Research Articles on Analysis of Illicit Drugs

hplc toxicology analysis

I have mentioned before on this blog that I am fascinated by the subject of toxicology and find peer-reviewed articles featuring the use of chromatography, such as the two articles featured here, absolutely riveting. By the way, did you know that the pace at which new and illicit psychoactive substances have been emerged has speeded up tremendously in the last few years?

Charged Aerosol Detection Speeds Analysis of Pharma Drug Impurities

carbocisteine impurity analysis

It has been a while since I have written on the analysis of impurities in drug formulations and, here, I am pleased to present a recently published peer-reviewed article featuring a validated HPLC and Charged Aerosol Detection method for measurement of impurities in carbocisteine. This drug is used as an anti-inflammatory mucolytic agent for the treatment of chronic obstructive lung disease and asthma. The European Pharmacopoeia monograph for the measurement of the impurity profile of cabocisteine (downloadable PDF) uses thin layer chromatography (TLC) along with ninhydrin derivatization. Unfortunately, not all related substances occurring through synthesis or caused by degradation are amino acids and are therefore not detected by this approach.

HPLC Speeds Analysis of 21 Preservative Compounds in Cosmetics

Analysis of Preservatives in Cosmetics

After the last post on the HPLC analysis of the toxic chemical rhodamine B in cosmetics (link to post), toxic chemicals in makeup have been on my mind a bit; and, when I found the application blogged here, I read it with great interest as it presented the analysis of preservatives, no less than 21 of them, in personal care products!

HPLC Method for Determination of Benzo(a)pyrene in Meat Products

Benzo(a)pyrene in smoked meat

Here, I am pleased to present an efficient HPLC method featuring an improved sample preparation method with fluorescence detection for the sensitive and rapid determination of benzo[a]pyrene (downloadable U.S. EPA factsheet) in complex meat products, such as sausages and preserved ham. 

HPLC Speeds Analysis of Rhodamine B in Cosmetics

analysis of cosmetics

I was fascinated by the HPLC application described in this post as it deals with chemicals, in particular Rhodamine B, (link to Wikipedia page), in cosmetics, and I am always curious to know what could be lurking in cosmetics that I might be using. Many regulatory bodies around the globe, such as the U.S. FDA (link to cosmetic labeling guide), European Commission (link to EU regulations on cosmetics), India's Bureau of Standards (link to regulations for cosmetics), and China's Food and Drug Administration (link to cosmetics regulatory pages) regulate the ingredients, testing, and labeling of cosmetics.

HPLC & CAD: Separation of Biochemical Buffering Agents

analysis of buffering agents

Good’s buffers are a series of 20 zwitterionic buffering agents that are used in biochemical experiments and HPLC mobile phases and feature stable pH control, low biological activity, low UV absorbance, and low metal affinity. This post features a newly released method separating 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions.

Hydrophobic Interaction Chromatography Method for MAb Analysis

analysis of papain digests

I am pleased to feature one of our hydrophobic interaction chromatography (HIC) columns (Thermo Scientific ProPac HIC-10 column) in a newly released method describing the separation of low-concentration species of an intentionally oxidized monoclonal antibody (MAb) from the native MAb. Those working in the field of developing MAbs as human therapeutics are fully aware of the challenges of working with MAbs, which can include various post-translational modifications and degradation during storage and delivery.

HPLC Speeds Determination of Dicyandiamide in Milk Powder

dicyandiamide analysis in milk powder

Here, I am pleased to feature an efficient and simple reliable HPLC method for the monitoring of dicyandiamide levels in milk and milk-derived products; last year, a New Zealand company found traces of this toxic chemical in its milk products (link to story).

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