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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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What Threatens your Heart More? Saturated Fat or Refined Carbohydrates?

  
  
  
  
  
  
fat content determination method

Like many others, I too have been following the ongoing story on the relationship between dietary fats and increased risk of cardiovascular disease not only from a personal standpoint but also from a chemist's perspective trying to find sample preparation solutions for challenging food matrices like extracting fat from processed foods like chocolate before analysis (link to downloadable PDF on method). In previous decades, the connection between dietary fats and increased risk of cardiovascular (link to peer-reviewed study) has been extensively studied and because of these studies, limiting saturated-fat consumption has been a long-standing dietary mantra for the past 30 years. But, recent meta-analyses of nearly two dozen studies, suggests that we may have picked the wrong culprit to reduce from our diets for decades. Processed carbohydrates, which many Americans eat today in place of fat, may increase the risk of obesity, diabetes, and heart disease more than fat does—a finding that has serious implications for new U.S. dietary guidelines expected this year.

Why I Turned from Phosphorylation to Glycan Analysis

  
  
  
  
  
  
glycosylation analysis by UHPLC

Most of my early research career focused on the post-translational modifications (PTMs) of proteins and in those days there was only one worthy PTM in my eyes and that was phosphorylation while glycosylation hardly registered. That was because phosphorylation governed important cellular processes (in my view at least) such as cell signalling; however, my love of phosphorylation slowly waned as I grew to understand the only reliable method to measure phosphorylation was with the potentially harmful radioactive Phosphorus-32 (32P)! (Link to Wikipedia page.) That’s when I started to get more interested in glycosylation and the huge diversity of modifications this could entail, after all phosphorylation was only adding phosphate on to a serine, threonine or tyrosine. Glycosylation offered more possibilities and intrigue and the opportunity to use other analytical techniques that didn’t involve 32P.

Glycan Analysis with a Particular Focus on the Immune System

  
  
  
  
  
  
Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

HPAE-PAD Analysis of Uronic Acids in Biofuel Biomass

  
  
  
  
  
  
analysis of Uronic Acids in Biomass

Did you know that the leaders in production of biofuels are the United States, France, Sweden, and Germany? And, the goal in the U.S. is to replace 75% of its imported oil by 2025 by alternative power sources including biofuels? (Source & Link Wikipedia).

HPAE-PAD Speeds Analysis of Carbohydrates in Beverages & Milk

  
  
  
  
  
  
analysis of carbohydrates in apple cider

The ongoing challenge for food regulatory bodies is not just identifying food contamination problems but also making sure that product formulation and product quality meets regulatory standards and food manufacturers report any ingredients that might impact immune-and allergy-sensitive individuals. By the way, did you know that India has banned the import of milk and milk products from China until June 2015 due to concerns of contamination of melamine? And. you can read about our solutions on the analysis of melamine in pet food and milk (link to post) on this blog.

Ion Chromatography Pathogen Research Studies

  
  
  
  
  
  
pathogen analysis using ion chromatography

Here, I am pleased to present two ion chromatography research studies on two common human pathogens, namely Streptococcus pneumoniae (pictured here) and Mycobacterium tuberculosis, that have become increasingly dangerous to humans as a result of their resistance to antibiotics. Indeed, the World Health Organization's (WHO) first global report on antibiotic resistance (link to report) has revealed a worldwide threat to public health and as per their findings this is a serious threat and potentially may affect anyone, in any region of the world. Without an urgent action plan, the world may be en route to a post-antibiotic era, where antibiotics may become ineffective and common infections and minor injuries may have the ability to kill again.

Chromatography and Sample Preparation Terminology Guide

  
  
  
  
  
  
chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

Chromatography Analysis of Additives & More in Beverages (II)

  
  
  
  
  
  
analysis of additives in beverage

After last month's post featuring chromatography applications for the analysis of sugars and artificial sweeteners in beverages (link to post), I am pleased to present two more application notebooks for the analysis of beverages, one for the analysis of carbonated beverages and the other for the analysis of additives in beverages.

Ion Chromatography Botany Research Studies

  
  
  
  
  
  
chromatography plant physiology studies

It has been a few months since I featured any chromatography methods in botany research studies, and here, I am pleased to present several peer-reviewed articles feature the use of our ion chromatography systems and columns.

Chromatography Analysis of Sugars & Sweeteners in Beverages (I)

  
  
  
  
  
  
chromatography sugars in beverages

Have you been following the increasing efforts by various governments to regulate the amount of sugar in beverages or impose sugar taxes on sugary drinks in an effort to reduce sugar in food and beverage products? In one story, UK's chief medical officer told a committee of MPs that a sugar tax may have to be introduced to curb obesity rates (link to story) and in another story, the state of Illinois, in the USA, is considering a new tax on sugary beverages (link to story). By the way, a new study concluded that fruit juice has only a bit less fructose than sugary sodas (link to study).

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