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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Why I Turned from Phosphorylation to Glycan Analysis

  
  
  
  
  
  
glycosylation analysis by UHPLC

Most of my early research career focused on the post-translational modifications (PTMs) of proteins and in those days there was only one worthy PTM in my eyes and that was phosphorylation while glycosylation hardly registered. That was because phosphorylation governed important cellular processes (in my view at least) such as cell signalling; however, my love of phosphorylation slowly waned as I grew to understand the only reliable method to measure phosphorylation was with the potentially harmful radioactive Phosphorus-32 (32P)! (Link to Wikipedia page.) That’s when I started to get more interested in glycosylation and the huge diversity of modifications this could entail, after all phosphorylation was only adding phosphate on to a serine, threonine or tyrosine. Glycosylation offered more possibilities and intrigue and the opportunity to use other analytical techniques that didn’t involve 32P.

Glycan Analysis with a Particular Focus on the Immune System

  
  
  
  
  
  
Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

Hydrophobic Interaction Liquid Chromatography: Sorting Through the Hype

  
  
  
  
  
  
Hydrophobic Interaction Liquid Chromatography or HILIC

In my 25 years in the chromatography industry, I have seen tremendous improvements in daily laboratory workflow through the years, in many cases, due to revolutionary products developed to save time and money for the researcher and provide more accurate and reproducible results day after day.  I know how difficult it is to stay current and sort through all the new developments in the industry especially while you’re trying to execute daily activities.  There is little time to explore all the new tools and techniques presented, and it’s hard to know which ones will benefit you. My goal, in this and future blog posts, is to discuss and provide resources on the latest tools and techniques that will help you improve and speed up your day-to-day work in the lab.

Isomeric Separation & Structural Identification of Labeled N-linked Glycans

  
  
  
  
  
  
HILIC glycan analysis

Analysis of glycosylation is a topic of growing importance in a number of fields such as antibody and biopharmaceutical characterization and human glycoproteome characterization for disease biomarkers. Variations in glycosylation have been implicated in a number of conditions including autoimmune disease, cancer and infectious disease as well as the correct functioning of the immune system, more details of which can be found in the review article by Zauner et al entitled, Glycoproteomic Analysis of Antibodies, (link to article).

HPLC & CAD: Separation of Biochemical Buffering Agents

  
  
  
  
  
  
analysis of buffering agents

Good’s buffers are a series of 20 zwitterionic buffering agents that are used in biochemical experiments and HPLC mobile phases and feature stable pH control, low biological activity, low UV absorbance, and low metal affinity. This post features a newly released method separating 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions.

Chromatography and Sample Preparation Terminology Guide

  
  
  
  
  
  
chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

Novel HPLC Approaches for Carbohydrate Analysis in Beverages & Food

  
  
  
  
  
  
Carbohydrate Analysis in Beverages & Food

Recently, we partnered with FoodProductionDaily.com to present a webinar on the use of universal Charged Aerosol Detection and selective pulsed amperometric detection for the direct determination of carbohydrates in complex food and beverage matrices. The advantages of two novel approaches, HPAE-PAD and HILIC-CAD was also discussed. The attendees asked several interesting questions on the types of detection and I thought they would make for good reading along with a couple of other resources.

Chromatography Software Workflow for Analysis of Unknown Glycans

  
  
  
  
  
  
hplc unknown glycan analysis

The technical note presented in this blog post presents a very interesting solution for the assignment of Glucose Units (GU) values to unknown glycans using hydrophilic interaction liquid chromatography (HILIC) separation, fluorescence detection, and Chromatography Data System (CDS) software.

Chromatography Data System: Five Webinars to Speed up your Analysis!

  
  
  
  
  
  
chromatography software

I am pleased to present a series of five upcoming webinars on features in our using our award-winning chromatography data system (CDS) software (Thermo Scientific Dionex Chromeleon CDS software)! The goal of the webinars is to present the CDS software features that permit chromatographers to get the analysis right first time!

Chromatography Solution Online Center Now Open!

  
  
  
  
  
  
liquid chromatography, ion chromatography, gas chromatography, and chromatography software

Do you know about our collaboration with Chromatographyonline.com in presenting an online Chromatography Solution Center to serve as a resource for chemists, scientists, and researchers by presenting the latest developments and applications, in chromatography?

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