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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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HPLC & CAD: Separation of Biochemical Buffering Agents

analysis of buffering agents

Good’s buffers are a series of 20 zwitterionic buffering agents that are used in biochemical experiments and HPLC mobile phases and feature stable pH control, low biological activity, low UV absorbance, and low metal affinity. This post features a newly released method separating 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions.

Chromatography and Sample Preparation Terminology Guide

chromatography terminology

In this very short blog post, I wanted to provide a link to a great resource, Advanstar Science's Chromatography and Sample Preparation Terminology Guide (link to page where you can click Register, fill out a brief form and then download the guide).

Novel HPLC Approaches for Carbohydrate Analysis in Beverages & Food

Carbohydrate Analysis in Beverages & Food

Recently, we partnered with to present a webinar on the use of universal Charged Aerosol Detection and selective pulsed amperometric detection for the direct determination of carbohydrates in complex food and beverage matrices. The advantages of two novel approaches, HPAE-PAD and HILIC-CAD was also discussed. The attendees asked several interesting questions on the types of detection and I thought they would make for good reading along with a couple of other resources.

Chromatography Software Workflow for Analysis of Unknown Glycans

hplc unknown glycan analysis

The technical note presented in this blog post presents a very interesting solution for the assignment of Glucose Units (GU) values to unknown glycans using hydrophilic interaction liquid chromatography (HILIC) separation, fluorescence detection, and Chromatography Data System (CDS) software.

Chromatography Data System: Five Webinars to Speed up your Analysis!

chromatography software

I am pleased to present a series of five upcoming webinars on features in our using our award-winning chromatography data system (CDS) software (Thermo Scientific Dionex Chromeleon CDS software)! The goal of the webinars is to present the CDS software features that permit chromatographers to get the analysis right first time!

Chromatography Solution Online Center Now Open!

liquid chromatography, ion chromatography, gas chromatography, and chromatography software

Do you know about our collaboration with in presenting an online Chromatography Solution Center to serve as a resource for chemists, scientists, and researchers by presenting the latest developments and applications, in chromatography?

UHPLC Methods for Fast Screening in Early Drug Discovery!

HPLC, UHPLC, HILIC, charged aerosol detection

We recently presented a very well-attended webinar on HPLC and UHPLC methodologies for versatile and powerful new pharma solutions featuring the use of our hydophilic interacton liquid chromatography (HILIC) and other reverse phase columns and our latest charged aerosol detector (Thermo Scientific Dionex Corona Veo Charged Aerosol Detector). I also found several excellent resources (an applications guides and two poster notes) to supplement the the information in the webinar and thought it would be useful reading for those working in the field of early drug discovery.

LC-MS/MS Study of Metabolomic Changes in Nematode C. elegans

metabolomic analysis

I found the HPLC and mass spectrometry metabolomics application blogged here fascinating as I had just read about the destructive impact of nematodes on food crops (link to story), and after receiving the application note, I was compelled to do some research on the Internet on the particular nematode studied in the application, namely Caenorhabditis elegans. (Isn't the image accompanying this post beautiful?)

Sample Preparation Simplified with New Syringe Filter Membranes

chromatography syringe filters

This is a very short blog post as I wanted to share the news on some new chromatography syringe filters that have been added to our range of syringe filter. 

HILIC Column Speeds Analysis of Anti-Cancer Chemotherapy Drug

impurity analysis in pharmaceuticals

Adding to our large library of chromatography pharmaceutical applications on this blog, I am pleased to feature an application note describing a simple and rapid HPLC method for the determination of decitabine and its α-anomer impurity. Decitabine (link to Wikipedia page) is an anti-cancer (antineoplastic or cytotoxic) chemotherapy drug and is used in the treatment of acute myeloid leukemia and myelodysplastic syndromes.

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