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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Isomeric Separation & Structural Identification of Labeled N-linked Glycans

HILIC glycan analysis

Analysis of glycosylation is a topic of growing importance in a number of fields such as antibody and biopharmaceutical characterization and human glycoproteome characterization for disease biomarkers. Variations in glycosylation have been implicated in a number of conditions including autoimmune disease, cancer and infectious disease as well as the correct functioning of the immune system, more details of which can be found in the review article by Zauner et al entitled, Glycoproteomic Analysis of Antibodies, (link to article).

Analytical Solutions for Regulatory Drinking Water Analysis

drinking water analytical methods

The access to clean, uncontaminated water has become one of the challenges of modern life around the globe as with increased industrial activities from developing countries, as well as the existing activities in developed countries with more and more industrial pollutants have been poured into nature and hence have negatively affected our water quality. Never before, have we had to pay so much attention to the quality of drinking, surface, and ground water that directly influence our daily lives. In addition, marine water monitoring and wastewater treatment has also become much critical in protecting human health.

Food Testing Using High Resolution LC-MS/MS: A Case Study at RIKILT

lc-ms ms pesticides analysis

Just watched a great 5 minute video which gave an overview on how high performance liquid chromatography (HPLC) coupled to High Resolution Accurate Mass (HRAM) mass spectrometry can help customers challenged with monitoring analytes such as pesticides and veterinary drugs in food and feed samples.

Fast Pharma Drug (Nevirapine) Impurity Profiling Using UHPLC-DAD

Pharma Drug Nevirapine Impurity Profiling

It has been a long while, almost two months, since I wrote about pharmaceutical drug analysis on this blog in post, titled, Charged Aerosol Detection Speeds Analysis of Pharma Drug Impurities (link to post), and I found the UHPLC application featured here while looking for something else on our website. I am pleased to write about this application because it features our latest UHPLC system (Thermo Scientific Vanquish UHPLC system) and can be used with our method transfer tool to speed and streamline the process of adapting HPLC parameters for UHPLC analysis.

Ion Chromatography: Case Study for Climate Change Studies

ice core climate change research

Did you know that the chemistry of ice cores can be preserved in samples that are hundreds of thousands of years old and can be analyzed by ion chromatography to reveal the climate of the past and to help predict future responses to continued global warming? These ice core samples are collected from deep within mountain glaciers and ice sheets found in remote places, such as Alaska and Greenland and are studied for their concentrations of major ions to develop a time scale. The majority of sample impurities can be traced to certain cations and anions which are measured, as stated earlier using ion chromatography.

Pesticides Analysis with High-Resolution Accurate Mass Spectrometry

high resolution mass spectrometry, HRAM, high resolution accurate mass, pesticide, veterinary drug, orbitrap, LC-MS, LC-MS/MS

Liquid chromatography with triple quadrupole mass spectrometry (LC-MS/MS) is considered the gold standard for screening and quantitation of pesticides in various food matrices. However, high-resolution accurate mass (HRAM) LC-MS/MS is growing in popularity, in part because it allows for retrospective analysis on additional analytes of interest and screening for unknown or unexpected pesticides is possible. This is because all ions can be collected and fragmented whereas triple quadrupole mass spectrometry requires a target list of compounds. Furthermore, method development time is often shorter by orders of magnitude with HRAM LC-MS/MS, enabling the screening of a large number of compounds compared to triple quadrupole mass spectrometers.

Charged Aerosol Detection Speeds Analysis of Botanicals

tcm analysis

Previously, this blog featured liquid chromatography analysis of Traditional Chinese Medicine (TCM) in a blog post that presented a HPLC applications notebook (link to blog post) on the subject; here, I discuss an HPLC application for the analysis of botanicals and natural products.

IC-MS Simplifies Complex Environmental Sample Analysis

chromatography water testing applications

Environmental sample analysis can be challenging since it requires both sensitive detection and the removal of any matrix interferences. Ion chromatography is the preferred separation technique for ionic species including inorganic anions/cations, small amines and organic acids, peptides and proteins, nucleic acids, and carbohydrates. The increasing demand for sensitivity leads to the emergence of a mass spectrometer (MS) with enhanced transmission efficiency for low mass ions as a powerful complementary detector to conductivity, UV and electrochemical detection.

Pomegranate Juice Fraud: HPLC Detects What's in Your Juice!

Pomegranate Juice adulteration testing

Did you know that a peer-reviewed study, titled, International multidimensional authenticity specification (IMAS) algorithm for detection of commercial pomegranate juice adulteration, (link to study), published in the J Agric Food Chem journal, found that only 35% of the commercially available pomegranate juice qualifies as authentic pomegranate juice?

Accelerated Solvent Extraction Environmental Technical Resource Guide

analysis of flame retardants

Did you know that the Accelerated Solvent Extraction (ASE) technique is accepted for use in U.S. EPA Method SW-846 3545A (downloadable PDF) for the extraction of a large number of herbicides, dioxins, furans, PAHs, PCBs, and diesel range organics? And, ASE is also accepted for use in U.S. EPA Method SW-846 6860 (downloadable PDF) for the determination of perchlorate and in CLP OLM 04.2A for semi-volatiles and pesticides.

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