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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Electrochemical Detection Troubleshooting

  
  
  
  
  
  
troubleshooting Electrochemical Detection (ECD)

I came across two technical notes on troubleshooting Electrochemical Detection (ECD) problems, featured in this blog post, while reading up on a recently released HPLC application that used this type of detection. And, soon after, I found a poster note on the topic and thought this would make for a useful read for those working in HPLC-ECD applications. For those new to this type of detection, ED is the most sensitive and selective mode of LC detection for the measurement of oxidizable or reducible compounds.

Ion Chromatography: Bromide Analysis in Hydraulic Fracturing

  
  
  
  
  
  
analysis of fracking water

I have written about our ion chromatography solutions for fracking in earlier posts (listed below) but while reading about some water analysis applications on our website, I came across a really interesting white paper on the analysis of bromide in fracking water that I am pleased to feature in this blog post. I am sure you have following the debate on the safety of fracking around the globe; just recently scientists in the province of Nova Scotia in Canada being the latest to weigh in by calling in for a 10-year moratorium on hydraulic fracturing (link to news story).

Scaling Down Sample Extraction for Limited & Volatile Sample Volumes

  
  
  
  
  
  
bioanalysis sample preparation

In an earlier post on the use of our recently released solid-phase extraction (SPE) micro elution plates (Thermo Scientific SOLA┬Á Solid Phase Extraction [SPE] Well Plates) for bioanalysis, I covered how these well plates can enhance sample concentration by 20X (link to post), and here I am pleased to present an application note on how these plates can be used to facilitate the scale down of an extraction method for use when sample volume is limited or volatile as in the case of bioanalytical samples.

Chromatography Analysis of Additives & More in Beverages (II)

  
  
  
  
  
  
analysis of additives in beverage

After last month's post featuring chromatography applications for the analysis of sugars and artificial sweeteners in beverages (link to post), I am pleased to present two more application notebooks for the analysis of beverages, one for the analysis of carbonated beverages and the other for the analysis of additives in beverages.

Creating An HPLC Affinity Titer Column for Monoclonal Antibody Analysis

  
  
  
  
  
  
monoclonal antibody analysis

In the poster presented in this blog post, scientists from our Sunnyvale (USA) research lab describe a straightforward method for creating designer assays for QC analysis of monoclonal antibodies (mAb) using our HPLC-compatible, streptavidin-coated monolith column. This affinity column produces narrow concentrated peaks with minimal diffusion, allows for titer analysis over a wide concentration range, and is applicable for a range of applications from small-scale protein to antibody titer analysis.

Pharma QA/QC Labs Look to LIMS for GLP, SOPs and QbD

  
  
  
  
  
  
Pharma QA/QC Labs rely on LIMS for QbD

While the paperless laboratory has been a topic of discussion for some time, the pace of adoption has never been faster. And since the collaborative landscape has changed dramatically in the past several years and increased the speed of discovery, alliances between commercial, academic and private research have become more complex.  These new layers of collaboration now require a different kind of integration of not just partners, but of inter-connected systems used by all of these collaborators. 

Ion Chromatography Botany Research Studies

  
  
  
  
  
  
chromatography plant physiology studies

It has been a few months since I featured any chromatography methods in botany research studies, and here, I am pleased to present several peer-reviewed articles feature the use of our ion chromatography systems and columns.

Sample Preparation & LC-MS Analysis: Pharma & Nanomaterials in Water

  
  
  
  
  
  
analysis of Nanomaterials in Water

Do you know that nanomaterials (down to 10,000 smaller than the diameter of a human hair) can be natural (viruses, wax crystals on a lotus leaf, spider silk, butterfly wing scales, blood, milk) or manufactured? (link and source Wikipedia.) Manufactured nanoparticles are increasingly being used in pharma drug delivery, disease detection, energy production, and even food and have triggered growing concern regarding their environmental behavior in aquatic environments. The ability to detect and quantify nanomaterials in complex water matrices has become an important issue (link to EU nanomaterials webpage).

GC-MS/MS Workflows for Metabolomics Experiments

  
  
  
  
  
  
gc-ms metabolite analysis

Did you know that there are up to 5 million metabolites (link to Wikipedia) in the plant kingdom? As a result, the identification and monitoring of these compounds can be extremely challenging. Here, I am pleased to present a couple of resources on the use of gas chromatography-mass spectrometry techniques that can increase the number of identifiable and selectively quantifiable metabolites by combining analytical methods for the different phases of the analysis. (By the way, previously I had written about the use of capillary ion chromatography and HR/AM for the metabolite profiling of oral cancers.)

Chromatography Analysis of Sugars & Sweeteners in Beverages (I)

  
  
  
  
  
  
chromatography sugars in beverages

Have you been following the increasing efforts by various governments to regulate the amount of sugar in beverages or impose sugar taxes on sugary drinks in an effort to reduce sugar in food and beverage products? In one story, UK's chief medical officer told a committee of MPs that a sugar tax may have to be introduced to curb obesity rates (link to story) and in another story, the state of Illinois, in the USA, is considering a new tax on sugary beverages (link to story). By the way, a new study concluded that fruit juice has only a bit less fructose than sugary sodas (link to study).

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