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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Automated Solid Phase Extraction & GC Analysis of Pesticides in Water

analysis of organophosphate pesticides

Did you know that the U.S. EPA posts annual reports on quality of drinking water by state? (Link to U.S. EPA site.) Does the regulatory entity responsible for monitoring water quality in your country do so? If yes, do share the link to its site in the Comments box below.

Charged Aerosol Detection Speeds Analysis of Pharma Drug Impurities

carbocisteine impurity analysis

It has been a while since I have written on the analysis of impurities in drug formulations and, here, I am pleased to present a recently published peer-reviewed article featuring a validated HPLC and Charged Aerosol Detection method for measurement of impurities in carbocisteine. This drug is used as an anti-inflammatory mucolytic agent for the treatment of chronic obstructive lung disease and asthma. The European Pharmacopoeia monograph for the measurement of the impurity profile of cabocisteine (downloadable PDF) uses thin layer chromatography (TLC) along with ninhydrin derivatization. Unfortunately, not all related substances occurring through synthesis or caused by degradation are amino acids and are therefore not detected by this approach.

Ion Chromatography Pathogen Research Studies

pathogen analysis using ion chromatography

Here, I am pleased to present two ion chromatography research studies on two common human pathogens, namely Streptococcus pneumoniae (pictured here) and Mycobacterium tuberculosis, that have become increasingly dangerous to humans as a result of their resistance to antibiotics. Indeed, the World Health Organization's (WHO) first global report on antibiotic resistance (link to report) has revealed a worldwide threat to public health and as per their findings this is a serious threat and potentially may affect anyone, in any region of the world. Without an urgent action plan, the world may be en route to a post-antibiotic era, where antibiotics may become ineffective and common infections and minor injuries may have the ability to kill again.

GC-MS/MS Provides Powerful Detection of Low-Level Alkylphenols

surfactant analysis using gc-ms

Here, I am pleased to feature a recently released GC-MS/MS method for the detection of  alkylphenol compounds (link to APE Research Council page) in food and environmental matrices.

HPLC Speeds Analysis of 21 Preservative Compounds in Cosmetics

Analysis of Preservatives in Cosmetics

After the last post on the HPLC analysis of the toxic chemical rhodamine B in cosmetics (link to post), toxic chemicals in makeup have been on my mind a bit; and, when I found the application blogged here, I read it with great interest as it presented the analysis of preservatives, no less than 21 of them, in personal care products!

HPLC Method for Determination of Benzo(a)pyrene in Meat Products

Benzo(a)pyrene in smoked meat

Here, I am pleased to present an efficient HPLC method featuring an improved sample preparation method with fluorescence detection for the sensitive and rapid determination of benzo[a]pyrene (downloadable U.S. EPA factsheet) in complex meat products, such as sausages and preserved ham. 

Gas Chromatography Environmental Microbiology Research Studies

gc-ms in microbiologyThe three peer-reviewed, environmental microbiology research studies presented briefly in this post all use our gas chromatography-mass spectrometry solutions for their research but I must admit that methane and bacteria came to my mind after I read these fascinating research stories tweeted by our Chromatography Solutions Twitter account (link to account):


Trust Your LIMS and Leave the Paper Behind in the R&D Lab


With fewer dollars to go around for R&D and increasing production costs, the life sciences industry must root out inefficiencies wherever possible and build in new processes to achieve improved productivity and greater efficiencies across an oftentimes much broader organization, starting with the laboratory. New technologies help accelerate discovery, which adds complexities that now-smaller staffs must manage.  Never has the need for comprehensive enterprise-wide information management and analysis been greater. A highly integrated Laboratory Information Management System (LIMS) can open the doors to new R&D investment.

HPLC Speeds Analysis of Rhodamine B in Cosmetics

analysis of cosmetics

I was fascinated by the HPLC application described in this post as it deals with chemicals, in particular Rhodamine B, (link to Wikipedia page), in cosmetics, and I am always curious to know what could be lurking in cosmetics that I might be using. Many regulatory bodies around the globe, such as the U.S. FDA (link to cosmetic labeling guide), European Commission (link to EU regulations on cosmetics), India's Bureau of Standards (link to regulations for cosmetics), and China's Food and Drug Administration (link to cosmetics regulatory pages) regulate the ingredients, testing, and labeling of cosmetics.

HPLC & CAD: Separation of Biochemical Buffering Agents

analysis of buffering agents

Good’s buffers are a series of 20 zwitterionic buffering agents that are used in biochemical experiments and HPLC mobile phases and feature stable pH control, low biological activity, low UV absorbance, and low metal affinity. This post features a newly released method separating 22 of these and other common buffer ingredients by simultaneous anion exchange (AEX), cation exchange (CEX), and hydrophilic interaction chromatography (HILIC) with simple isocratic conditions.

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