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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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Guiding the Sweet Revolution in Glycomics

  
  
  
  
  
  
HPAE-PAD mass spectrometry analysis of glycoproteins

There’s been a lot of interest recently in the study of glycans and glycoforms, such as proteins and peptides that have glycans attached to them. The analysis of glycans (also known as carbohydrates, or sometimes simply sugars) is of growing importance to sciences as diverse as pharmaceutical drug development, cancer research, stem cell research and biofuels development.

How Are You Controlling Copper Additive Performance in Electroplating Baths?

  
  
  
  
  
  
semiconductor chips

Getting fast, accurate measurements on the performance of additives used in semiconductor electroplating baths is of critical importance in providing plating quality which includes uniformity, brightness, and consistency. Liquid chromatography with charged aerosol detection (CAD) and electrochemical detector techniques ensures reliable plating by detecting individual components, their byproducts, and other components in process monitoring applications. The technique has been successfully applied to the determination of individual organic additives in acid copper and nickel plating baths.

Sifting Through Bromate Analysis Methods for Drinking Water Analysis

  
  
  
  
  
  
determination of trace concentrations of bromate and oxyhalides in high-ionic-strength matrices

Much has been written on bromate methods (link to article on comparison of regulated methods) over the years, but what still confuses some people are the multitude of methods available for the analysis of bromate. As a well known toxic disinfection by-product typically formed from the ozonation of water containing bromide, bromate is regulated in many countries around the world. The U.S. Environmental Protection Agency (EPA) has established a regulatory maximum contaminant level (MCL) of 10 µg/L of bromated in drinking water and a maximum contaminant level goal (MCLG) of  0.8 µg/L. The World Health organization (WHO) also recommends an MCL of 10 ug/L of bromated in their publication, titled, Bromate in Drinking Water, (downloadable PDF). The Japanese Ministry of Health, Labor and Welfare has a similar level. In addition, the European Commission established a lower MCL of 3 μg/L bromate in natural mineral waters and spring water treated by ozonation.

Honey Laundering: A Food Fraud That’s Not So Sweet

  
  
  
  
  
  
honey fraud testing

I love the term honey laundering, and in this continually growing food fraud scandal wouldn’t it be wonderful, almost romantic, if it were actually the bees that were perpetrating the crime and making all the money? I took some time to look into the background of honey adulteration, literature and analytical methods, specifically an isotope ratio mass spectrometry (IRMS) method which I know is used routinely for product authentication in the food safety testing market.

Will Strontium Get on the Final Contaminant List for Drinking Water?

  
  
  
  
  
  
strontium contamination in drinking water

Talking about drinking water regulations, you may have heard that the U.S. EPA made a preliminary determination for a strontium regulation in drinking water (link to the announcement) on October 20, 2014. The EPA planned to collect comments on the preliminary determination by December 19, 2014 and by the end of 2015, the EPA will have made the final decision if strontium, as a metal analysis component, will get on the primary drinking water contaminant list (link to EPA drinking water page). Once the final approval is made, within 24 months, the EPA will come up with the maximum contaminant level goal (MCLG) for strontium in drinking water and within another 18 months, the EPA will finalize the regulation.

Breeding Program & Pesticides Use Propel Strawberries to Celebrityhood

  
  
  
  
  
  
pesticides strawberries

Strawberries are well on their way to becoming California’s newest celebrity as indicated by the two strawberries-related stories that have been much in the news in California this year. First, there have been serious disagreements between a university and the scientist running the university’s strawberry breeding program to the extent of the media labeling it as a feud (see links to stories below). The second debate formed around the use of certain pesticides in California. This led to California’s Department of Pesticide Regulation establishing the the nation’s strictest limits on chloropicrin, a pesticide commonly injected into soil before strawberries are planted. (Links to the California Department of Pesticide Regulation page and their chloropicrin page.) Both stories are causing much interest and concern in the strawberry-growing community.

Rapid Fruit Juice Analysis: A Case Study at the J.M. Smuckers Co.

  
  
  
  
  
  
analysis of glucose, fructose, and sucrose in jams purees

What does it take in terms of process analysis and control to manufacture jams, purees, and fruit juice from fresh, sweet summer fruits? Historically, wet chemistry methods have been been used to analyze raw materials and fruit juice products in a manufacturing setting, but this is a slow tedious process and for labs requiring a high turnover of samples is not efficient or timely.

The European Bioanalysis Forum: What’s Going on With Antibodies?

  
  
  
  
  
  
antibody conference in barcelona

This week I encountered the power of nature. Because of weather on the East coast of the USA, my flight was cancelled and I missed a conference I planned to attend in Washington DC about the characterization of biotherapeutics. I really hope that the majority of attendees manage to make it.

Your Elemental Analysis is Costing You More Than You Think: Part 1

  
  
  
  
  
  
inductively coupled plasma optical emission spectrometry

In this three-part series blog post on trace elemental analysis, I will discuss key focus areas for a laboratory intent on maximizing its productivity and improving its profitability. In this first post, I will discuss the use of a switching valve to conduct elemental analysis and describe the use of a segmented stream during sample analysis and highlight its advantages over those when a continuous flow stream is employed. 

Why I Turned from Phosphorylation to Glycan Analysis

  
  
  
  
  
  
glycosylation analysis by UHPLC

Most of my early research career focused on the post-translational modifications (PTMs) of proteins and in those days there was only one worthy PTM in my eyes and that was phosphorylation while glycosylation hardly registered. That was because phosphorylation governed important cellular processes (in my view at least) such as cell signalling; however, my love of phosphorylation slowly waned as I grew to understand the only reliable method to measure phosphorylation was with the potentially harmful radioactive Phosphorus-32 (32P)! (Link to Wikipedia page.) That’s when I started to get more interested in glycosylation and the huge diversity of modifications this could entail, after all phosphorylation was only adding phosphate on to a serine, threonine or tyrosine. Glycosylation offered more possibilities and intrigue and the opportunity to use other analytical techniques that didn’t involve 32P.

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