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A blog on the latest applications, articles, & research on chromatography solutions in sample preparation, Ion Chromatography (IC),
High Pressure Liquid Chromatography (HPLC), Ion Chromatography-Mass Spectrometry (IC-MS), Gas Chromatography (GC),
Gas Chromatography-Mass Spectrometry (GC-MS and GC-MS/MS), and software (Chromeleon CDS, LIMS, and ProteinCenter).

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A Milestone in Pesticides Analysis for Orbital Trap Mass Spectrometry


Testing Total Acids in Wine Using Automated Photometric Methods

analysis of tartaric acid in wine samples

Here, in my first post on the advantages of using discrete photometric analyzers, I will be addressing the challenges of testing wine elements that contribute towards the taste of a good wine. Typically, wine drinkers look for a robust mouth experience which is the result of amount of acidity in wine, one of its most appealing characteristics and which complements foods in a palate-cleansing manner (link to page on If a wine is too low in acid, it tastes flat and dull; too high in acid and it is tart and sour. Natural acids, such as tartaric, malic, and citric have the freshest, purest acid tastes. Fermentation acids, like lactic and acetic, add milder, complex flavors. Since acidity greatly influences the taste of wine, winemakers need to understand the role that each of the various acids plays during fermentation and production to ensure an end product with great lasting flavor. Common methods for determination of acids can be accessed in AOAC Official Methods of Analysis 945.08, 950.15, 962.12 (19th edition).

Our New Year’s Resolution: More Resolutions to Your Laboratory Challenges

analytical chemistry blog

Every year brings changes that affect us both personally and professionally. It seems that technology moves faster and faster and it can be hard to keep up, which can be both exciting and a bit overwhelming. This is especially true for scientists as techniques like chromatography, mass spectrometry, elemental analysis, and sample preparation – not to mention software – continue to make significant advances. As a chemist, researcher, or lab manager, you may have had to deal with some of the following challenges in 2014:

Australia Recalls Tonic Water – The Hazards of Food Mislabeling

analysis of quinine in tonic water

Earlier this month I read that Australia has recalled Indian Tonic Water (link to story) as some of the tonic water bottles were mislabeled as soda water and, hence, the labels did not include information on presence of quinine in the beverages; both Australia and the EU require that the presence of quinine in such beverages be declared. Indeed, the UK's SCHEDULE 8: MISLEADING DESCRIPTIONS states that "The name “Indian tonic water” or “quinine tonic water” shall not be applied to any drink unless the drink contains not less than 57 mg of quinine (calculated as quinine sulphate B.P.) per litre of the drink.

Pharmaceutical USP 232 Chapter Revisions & Harmonization with ICH Q3D

trace element determination in APIs

As practitioners in the world of pharmaceutical manufacturing and quality control, you’ll know that the U.S. Pharmacopeial Convention (USP) 232 and its requirements for trace element determination in APIs, excipients and all other materials that make their way into, or are in contact with your finished products is on the horizon. The implementation date for meeting the demands of this regulation, as set by the U.S. Pharmacopeial Convention, and its companion protocol, USP 233, was, until last week, 1st December 2015, but on 14th January this year, the USP announced that this date was to be pushed back to 1st January 2018. The final specifics of USP 232 have yet to be agreed upon, with further changes having been recently (October 2014) proposed for elemental impurities, with the aim of aligning USP 232 with its European counterpart guidelines, as described in the publication, Guideline for Elemental Impurites Q3D, (downloadable PDF) and referred to as ICH Q3D. 

Is Suppressed Conductivity for Cation Analysis by IC needed? YES!

wastewater analysis by ion chromatography

For many years, I’ve witnessed a very confusing aspect about cation analysis by ion chromatography. In some circles, there is a myth that suppression isn’t needed. No one would consider NOT using a suppressor for anion analysis, so why then would the opposite be true for cation analysis?

Arsenic Speciation in Rice: Fast & Simple Analysis Using IC-ICP-MS

inorganic arsenic speciation in rice

In my previous blog titled: Arsenic Speciation: The Media and the Science (link to blog post) I promised updates on this hot topic and as a big plus for me personally, this review incorporates my passion for ion analysis. My previous discussion points highlighted the importance of measuring the inorganic arsenic species, commenting on upcoming regulations and the ongoing research at the Institute for Global Food Security at Queens University Belfast (QUB) (link to website) and here I wanted to share the very latest analytical method on the subject published just last month.

Sample Preparation: Three Techniques Reviewed

hplc sample preparation

I hope you found the last installment on Hydrophobic Interaction Liquid Chromatography (link to blog post) useful. Supporting my goal to discuss and provide resources on the latest tools and techniques that will help improve and speed up your day-to-day work in the lab, I will discuss sample preparation for HPLC in this post.

Troubleshooting Pharmaceutical Assays in HPLC & UHPLC

troubleshooting pharmaceutical HPLC and UHPLC applications

The focus of this post to present several resources for troubleshooting UHPLC and HPLC methods in pharmaceutical and biopharmaceutical applications. How this post came about was with my receiving the first resource via an email and then learning about the webinar.

Glycan Analysis with a Particular Focus on the Immune System

Human Immune System attack a virus

As an immunologist and analytical scientist, I have always been fascinated with the immune system and how pathogens and the immune system have evolved hand-in-hand as the former seeks new ways to avoid and disguise itself from the latter and the immune system develops alternative mechanisms to seek out and destroy invading pathogens. It’s a miniature arms race occurring inside each and every one of us, but only really becoming noticeable to us when we feel the effects of the raging battle through illness. I do believe that the immune system represents a model and spell-binding system in which to view evolution occurring, although my young children would probably disagree and much prefer an alternative bedtime story to one of my favorite books for children, titled, Your Amazing Immune System! (details of how to get a free copy of this book if you wish to go with the immunological children’s bedtime story.)

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